Image mounter macro notes
v. 1 11.11.98
A new digital scanning microscope (Philips XL-30) has recently become
available at the SEM unit at the Natural History Museum. This new device
is able to capture digital high resolution images (1.3 MB, 1424x968
pixel, 150dpi) as well as normal photomicrographs. For archiving and
filing reasons a macro has been written that automatically mounts and
prints four images on one page.
System requirements
Macintosh or IBM compatible computer, NIH-image (freeware available
for both platforms at http://rsb.info.nih.gov/mih-image/)
Procedure
Open NIH-image and load macro image mounter.
Open one image of the stack you want to assemble.
Most SEMs produce distorted images (pixel aspect ratio Ð 1). Run
initialise as described below, but set scaling factor to one. Afterwards
run macro correct XL30 images. The pixel aspect ratio used here work with
our XL30-framegrabber combination. You can adjust the values in the macro
file. The macro replaces the saved versions of the images with a new,
corrected version without asking!
To print the images:
Set printer default to landscape.
Run macro initialise
The filepath will be displayed. Enter the index number which is
attributed to the images. We usually have picture numbers like 99-1.TIF,
where 99- resembles the index number and 1 is the running number of the
picture. This file information will be displayed in the lower left corner
of every image on one page.
Enter running number of the first and the last image to be mounted.
Enter the scaling factor for the images. Note that NIH-image always
scales down the active window to the papersize before printing. We
usually use a scaling factor of 0.4 to 0.6 which offers the best
resolution for dysub and laser printers.
If your SEM produces images with a pixel-aspect ratio of 1 then use macro
combine and print mosaic. This macro will mount and print your
images on the specified printer.
In case of problems please contact
Markus Geisen
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This page is based on work primarily of Markus Geisen
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